||The positive dissolution of the trace element ion from the alloy for prosthetic dentistry is expected in Regenerative Dentistry. Vascularization, which carries oxygen and nutrition, is indispensable to tissue generation. We investigated the influence of the human skin model on cell viability test and the formation test of tubule-like structures be very low concentration copper, titanium and zinc ions. In both experiments, the influences of metal ions significantly differed. The cell viability test using a human skin model showed significant differences in the reaction to slight cell stimulate on. In testing the formation of tubule-like structures, the influence of the singularity of HUVEC should be considered. In the dentistry field especially, the dissolution of chemical substances from material, applications in reparative dentistry from prosthetic dentistry material to saliva is expected. The development of technology to control the dissolution of metal ions is also required. Basic experiments focusing on specific metal ions or changes in the exposure period need to be accumulated in future studies.