|発表タイトル||The traditional Chinese medicine improbes the expression of adipocytokine mRNA in simple obesity mouse|
|学会名||The 4th Sino-Japanese Conference on Stomatology 2008|
|発表者・共同発表者||Ikeo T, Kamada A, Yoshikawa Y, Domae E, Goda S, Tamura I, Takaishi Y|
|学会抄録||「Program & Abstracts of the 4th Sino-Japanese Conference on Stomatology 2008」 pp.91
「Program & Abstracts of the 4th Sino-Japanese Conference on Stomatology 2008」 pp.91
|概要||Objective: Adipose tissue is now known to secrete some adipocytokines, which are supposed to play a role in regulation of energy homeostasis. In this study, we evaluated effects of administration of Traditional Chinese Medicine (TCM) on adipocytokine expression in adipocytes of simple obesity mice.
Methodology: Three-week old male mice were used in this study. Obesity mice induced by high fat diet (HFD) for 10 weeks were administered with TCM daily for 6 weeks. Subcutaneous white adipose tissue (SAT), visceral adipose white tissue (VAT) and interscapular brown adipose tissue (BAT) were extirpated from HFD group, TCM group and normal diet control group. Total RNA was extracted from the adipose tissues, and the mRNA expression level was measured using real-time quantitative PCR technique.
Results: The increase in body weight of obesity mice was suppressed by the administration of TCM. Leptin mRNA expression level in all adipose tissues of HFD group significantly increased compared with those of control group. UCP1 mRNA expressed only in BAT, and it was also significantly increased by obesity. Though in control group, TNF- mRNA expression was only detected in SAT as a trace, it increased significantly in all tissues of HFD group. In contrast, both mRNA expression levels of adiponectin and adipsin in all adipose tissues of HFD group significantly decreased compared with those of control group. TCM treatment helped the decreases of adiponectin and adipsin levels and the increases of leptin and TNF-, although the treatment did not influence the expression of PPAR and UCP1.
Conclusion: It is indicated that administration of TCM may not influence the adipocyte differentiation and energy production, but may be useful to improve insulin resistant and diabetes and to control obesity therapies.