|発表タイトル||Methyl-β-cyclodextrin suppresses osteoclastogenesis by modulating the MAPKs signaling pathways|
|学会名||The 4th Sino-Japanese Conference on Stomatology 2008|
|発表者・共同発表者||Domae E, Goda S, Ogawa Y, Takeuchi S, Yoshikado R, Sakata T, Yoshikawa Y, Tamura I, Kamada A, Yamamoto K, Morita S, Ikeo T|
|学会抄録||「Program & Abstracts of the Sino-Japanese Conference on Stomatology 2008」 pp.95
「Program & Abstracts of the Sino-Japanese Conference on Stomatology 2008」 pp.95
|概要||Objectives: Bone is continuously remodeled by bone resorption and formation. The bone metabolism is tightly regulated to maintain homeostasis. Deviation from the normal conditions of bone resorption would result in bone diseases. Lipid rafts are specialized plasma membrane microdomains enriched with glycosphingolipids, Sphingomyelin and cholesterol. Lipid rafts are important for the transport of selected membranes and relay stations in intracellular signaling.
Methodology: To investigate the role of lipid rafts in RAW264 cell signaling, lipid rafts were disrupted by depleting cholesterol with methyl-β-cyclodextrin (MβCD)
Results: We found that RANKL-induced differentiation into osteoclasts was markedly inhibited by MCD in a dose-dependent manner. In contrast, MβCD enhanced the phosphorylation of ERK1/2 MAP kinase.
Conclusion: These data suggest a crucial role for cholesterol in the regulation of the RANKL-mediated signaling pathway and osteoclast differentiation in RAW264 cells, which reflects its importance in the formation of plasma membrane lipid rafts.