||Influence of adipocytokines on differentiation of pro-osteoblastic cells
||Journal of Oral Tissue Engineering
||Kamada A, Ikeo T, Yoshikawa Y, Domae E, Goda S, Tamura I, Kon-I H, Okazaki J, Kawamoto A, Komasa Y, Toda I, Suwa F
||Adipocytokines are a group of adipocyte-derived biologically active molecules, including adiponectin, leptin, tumor necrosis factor-alfa (TNF-alfa), resistin. A proteolytic cleavage product of adiponectin/Acrp30, gAcrp30, is found to circulate in plasma and to have a three-dimensional structure similar to that of TNF-alfa. In this study, we demonstrated the expression of adiponectin receptors in cultured pro-osteoblastic cells, and investigated the influences of gAcrp30 and TNF-alfa on osteoblast differentiation.
RT-PCR analysis demonstrated the expression of transcripts for both adiponectin receptors, AdipoR1 and AdipoR2, in murine pro-osteoblastic cells MC3T3-E1. Both gAcrp30 and TNF-alfa suppressed mRNA levels of type I collagen and RANKL compared with time-matched controls. Interestingly, gAcrp30 had no significant influence on the promotion of osteocalcin expression induced by the stimulation of ascorbic acid and beta-glycerophosphate, although TNF-alfa inhibited the increase of osteocalcin expression.
These results indicated that adiponectin may not affect osteoblast differentiation but may suppress osteoclastogenesis in an endocrine manner, while TNF-alfa may inhibit the differentiation of pro-osteoblastic cells.