||Effect of Infusion as a Cell Preservation Solution on HPLFSs
||The 97th General Session of the IADR, will be held in conjunction with the 48th Annual Meeting of the AADR and the 43rd Annual Meeting of the CADR
||Okamura T, Takeuchi T, Ikeda C, Tominaga K
||Vancouver, British Columbia, Canada
||• HPLFs were seeded in 96-well plates. These cells were cultivated in Dulbecco's modified Eagle's medium (DMEM) containing 10% fetal bovine serum (FBS) supplemented with 500U/mL penicillin and 500µg/mL streptomycin under 5% CO2 conditions until confluent. In order to approximate the osmotic pressure of the living body in the preservative solution, bicarbonate ringer solution was used. 24h after cell seeding, the culture medium was removed and preserved in bicarbonate ringer solution and in physiological saline as a control group. HPLFS was preserved (37°C) for the experimental groups, and at room temperature (RT; 20 to 25°C) for the control group. Physiological saline and bicarbonate ringer solution were used as preservative solutions. In addition, ceftriaxone sodium and fosfluconazole were added to each infusion. Living cells were stained with calcein-AM and dead cells were stained with EthD-III. They were observed morphologically using a confocal laser scanning microscope (CLSM) after being preserved for 1h in each solution.
Living cells were stained with Calcein-AM and dead cells were stained with EthD-III. They were observed morphologically using a confocal laser scanning microscope after being preserved for 1h in each solution.