||The role of syndecans in the human dental pulp.
||Journal of Oral Tissue Engineering
||Tsuda K, Kamada A, Yoshikawa Y, Domae E, Nishiura A, Matsumoto N
||Objectives: Syndecans (SDCs) are a family of cell surface heparan sulfate proteoglycans known to play critical roles in regulating multiple cellular functions, including participation in inflammation and wound healing as a host defense molecule. The present study was undertaken to analyze the role of syndecan family in human dental pulp cells and the effect of an inflammatory cytokine.
Materials and methods: Human dental pulp cell cultures from impacted third molars were established. Cells were cultured with the inflammatory mediator interleukin-1beta (IL-1beta). The gene expressions of SDCs were analysed by quantitative RT-PCR.
Results and significance: The relative level of mRNA expressions for SDC1 and SDC2 increased with cell proliferation, but both of them were suppressed significantly by IL-1beta stimulation. The expression of SDC3 remained unaltered by IL-1beta, although the expression level for SDC4 showed a rapid and strong increase. SDC4 was shed into the culture medium in a process involving MMPs. Shedding of SDC4 was increased after IL-1beta-stimulation. Our data demonstrate that all of SDC family express in human dental pulp cells and play different roles, and, most notably, SDC4 is involved in acute inflammation.