||Effectiveness of calcium hydroxide on LPS-induced hard tissue resorption
||Journal of Osaka Dental University
||Shida M, Baba T, Kon-I H, Taguchi Y, Hayashi H
||Although the application of calcium hydroxide in the root canal is reportedly effective for the prevention of external root resorption, details of its mechanism of action are unclear. We studied the effect of calcium hydroxide on the expression of lipopolysaccharide(LPS) induced hard tissue resorption-related genes in human osteoblastic cells (Saos-2) to evaluate how it prevented hard tissue resorption. The effects of calcium hydroxide on the expression of genes of TNF-a, IL-6, OPG and RANKL expressed by LPS-stimulated human osteoblastic cells were evaluated using the real-time reverse transcriptase-polymerase chain reaction. The data were compared employing Student’s t-test (n=6) at the significance level of 0.05. Calcium hydroxide inhibited the expression of proinflammatory cytokines induced by LPS. It also inhibited the expression of RANKL, which is always expressed in human osteoblastic cells. Calcium hydroxide appeared to inhibit LPS-induced inflammatory hard tissue resorption.